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event:electrophoresis [2013/09/21 15:22]
kyknos [Where's the catch?]
event:electrophoresis [2013/09/21 15:23] (current)
kyknos [Where's the catch?]
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   * UV light source (alternatively blue LEDs should be sufficient, let's try it out!)   * UV light source (alternatively blue LEDs should be sufficient, let's try it out!)
   * other auxiliary lab equipment: gloves, beakers...   * other auxiliary lab equipment: gloves, beakers...
 +  * TAE buffer in a sufficient quantity (Kyknos and Bluebear) - we have NaOH, EDTA, TRIS, acetic acid and distilled water - TAE tested succesfully with 60V power supply and diy loading dye (sucrose+cresol red)
  
 What we need: What we need:
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     * technical discussion: http://brmlab.cz/pipermail/brmlab/2013-September/005533.html     * technical discussion: http://brmlab.cz/pipermail/brmlab/2013-September/005533.html
     * possible inspiration: http://www.instructables.com/id/Gel-electrophoresis-power-supply/     * possible inspiration: http://www.instructables.com/id/Gel-electrophoresis-power-supply/
-  * loading dye, EtBr ([[user:chido]] and Iva will provide these) +    * loading dye, EtBr ([[user:chido]] and Iva will provide these) 
-  * TAE buffer in a sufficient quantity (Kyknos and Bluebear) - we have NaOH, EDTA, TRIS, acetic acid and distilled water - TAE tested succesfully with 60V power supply and diy loading dye (sucrose+cresol red) +  
  
 ===== Useful and fun links ===== ===== Useful and fun links =====
   * Try out how DNA gel electrophoresis works online: http://learn.genetics.utah.edu/content/labs/gel/   * Try out how DNA gel electrophoresis works online: http://learn.genetics.utah.edu/content/labs/gel/
   * For the power supply hackers, here's a commercial power supply and it's parameters: http://www.bio-rad.com/en-cz/product/powerpac-basic-power-supply   * For the power supply hackers, here's a commercial power supply and it's parameters: http://www.bio-rad.com/en-cz/product/powerpac-basic-power-supply
 
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