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event:electrophoresis [2013/09/21 15:22]
kyknos [Where's the catch?]
event:electrophoresis [2013/09/21 15:23] (current)
kyknos [Where's the catch?]
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   * UV light source (alternatively blue LEDs should be sufficient, let's try it out!)   * UV light source (alternatively blue LEDs should be sufficient, let's try it out!)
   * other auxiliary lab equipment: gloves, beakers...   * other auxiliary lab equipment: gloves, beakers...
 +  * TAE buffer in a sufficient quantity (Kyknos and Bluebear) - we have NaOH, EDTA, TRIS, acetic acid and distilled water - TAE tested succesfully with 60V power supply and diy loading dye (sucrose+cresol red)
  
 What we need: What we need:
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     * technical discussion: http://​brmlab.cz/​pipermail/​brmlab/​2013-September/​005533.html     * technical discussion: http://​brmlab.cz/​pipermail/​brmlab/​2013-September/​005533.html
     * possible inspiration:​ http://​www.instructables.com/​id/​Gel-electrophoresis-power-supply/​     * possible inspiration:​ http://​www.instructables.com/​id/​Gel-electrophoresis-power-supply/​
-  ​* loading dye, EtBr ([[user:​chido]] and Iva will provide these) +    ​* loading dye, EtBr ([[user:​chido]] and Iva will provide these) 
-  ​* TAE buffer in a sufficient quantity (Kyknos and Bluebear) - we have NaOH, EDTA, TRIS, acetic acid and distilled water - TAE tested succesfully with 60V power supply and diy loading dye (sucrose+cresol red) +  ​
  
 ===== Useful and fun links ===== ===== Useful and fun links =====
   * Try out how DNA gel electrophoresis works online: http://​learn.genetics.utah.edu/​content/​labs/​gel/​   * Try out how DNA gel electrophoresis works online: http://​learn.genetics.utah.edu/​content/​labs/​gel/​
   * For the power supply hackers, here's a commercial power supply and it's parameters: http://​www.bio-rad.com/​en-cz/​product/​powerpac-basic-power-supply   * For the power supply hackers, here's a commercial power supply and it's parameters: http://​www.bio-rad.com/​en-cz/​product/​powerpac-basic-power-supply
 
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